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To explore the relationship between telomere length changes and age, and to provide data and reference for further study of geriatric medical problems. Methods: The healthy people over 20 years old were chosen as subject from several hospitals by random sampling method, and their peripheral blood samples were collected. The relative length of telomere was detected by quantitative real-time PCR, and the relationship between age and telomere length was analyzed by statistical software. Results: A total of 1 022 samples were obtained. There were significant differences in the relative telomere length among different age groups (F=21.492, P<0.001). Telomere length and age showed negative correlation (r=-0.325, P<0.001), the regression equation was y=-0.008x+1.772 (x for age, y for the average telomere length, P<0.001). Conclusion: The telomere length for peripheral blood leukocytes in healthy people varies between different age groups, suggesting that telomere length gradually decreases with age.
Subject(s)
Adult , Humans , Young Adult , Age Factors , Leukocytes, Mononuclear , Regression Analysis , Telomere , GeneticsABSTRACT
Objective:To build a social aging scale for healthy people,and get the accuracy and feasibility of the scale to judge the aging of healthy people on the basis of the empirical research.Methods:Stratified random sampling was conducted according to the nine different age groups,and the age-related social function information was collected for healthy people by using the scale,which was made in our own plant;the distribution of the score,the correlations between the score and age or telomere length were analyzed by statistical software.Results:A total of 2 297 subjects were collected for the analysis.The ratio of male to female was 0.8:1.Social function of aging score was in the range of 3.00-12.90;aging score of personal ability was in the range of 1.00-4.45;aging score of social participation was in the range of 1.00-4.52;and aging score of organizational communication was in the range of 1.00-5.00.The correlation coefficient of aging scores with age was 0.696 (P＜0.001).The result of telomere length showed that the relative telomere length was 1.056±0.261.The telomere length in different groups were significantly different (F=35.803,P＜0.001).The telomere length was negatively correlated with aging (r=-0.964,P＜0.001) and social function of aging (r=-0.857,P＜0.001),respectively.Conclusion:The scores of age-related social function,personal ability,social participation,and organizational communication are increased with age.The telomere length is negatively correlated to aging score.The scale is applicable to measure social function of aging,which can provide reference for evaluation of the comprehensive of aging.
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OBJECTIVE@#To build a physiological aging scale for healthy people. @*METHODS@#We collected age-related physiologic items through literature screening and expert interview. Two rounds of Delphi were implemented. The importance, feasibility and the degree of authority for the physiological index system were graded. Using analytic hierarchy process, we determined the weight of dimensions and items. @*RESULTS@#Using Delphy mothod, 17 physiological and other professional experts offered the results as follow: coefficient of expert authorities Cr was 0.86±0.03, coordination coefficients for the first and second round were 0.264(χ2=229.691, P<0.001) and 0.293(χ2=228.474，P<0.001), respectively. The consistency was good. The aging scale for healthy people included 3 dimensions, namely physical form, feeling movement and functional status. Each dimension had 8 items. The weight coefficients for the 3 dimensions were 0.54, 0.16, and 0.30, respectively. The Cronbach's α coefficient of the scale was 0.893, the reliability was 0.796, and the variance of the common factor was 58.17%. @*CONCLUSION@#The improved Delphi method or physiological aging scale is satisfied, which can provide reference for the evaluation of aging.
Subject(s)
Humans , Aging , Physiology , Delphi Technique , Reproducibility of ResultsABSTRACT
0bjective To investigate the pattern of disease relapse and prognostic risk factor of patients with cervical carcinoma and pelvic lymph node metastasis.Methotis A total of 124 cases of International Federation of Gynecology and Obstetricfi(FIG0)I bl-Ⅱ a cervical carcinoma with pelvic node metastasis who were treated at the Cancer Center of Sun Yat-sen University during January 1994 to December 2001 were selected for this study.Prognosis and recurrence were retrospectively analyzed using the clinico.pathological data.Results The overall 5 year flun,ival and 5 year disease-free survival were 63.3% and 61.4%.respectively.Overall recurrence rate was 39.5%(49/124),among which intra-pelvic relapse (61.0%,25/41)was significantly more common than extra-pelvic relapse(31.7%,13/41;P=0.008).Multivariate analysis identified involvement of common iliac node as an independent prognostic factor(P=O.035).According to this factor,node-positive patients could be divided into low risk group(without common iliac node involvement,104 cases)and hiSh risk group(with common iliac node involvement,20 cases).The 5 year disease-free survival were 69.4%and 24.5%respectively,with a significant difference(P=0.003).Intra.pelvic relapse was observed in 22.1%(23/104)of low risk and 25.0% (5/20)of high risk group respectively,with no significant difference(P>0.05).However extra-pelvicrelapse wag seen in 7.7%(8/104)of low risk and 40.0%(8/20)of hish risk group,with a significant difference(P<0.01).Conclusions Common iliac node involvement is a significant factor influencing the prognosis of patients with cervical carcinoma and pelvic lymph node metastasis.Patients with positive common iliae nodes have significantly decreased 5 year disease.free survival and hishcr extra-pelvic disease recurrence rates compared with those whose common iliac nodes are negative.These findings provide impo.rtant data for design of individualized treatment mode.